#Facsdiva Tutorial Download Word File#

Use these tutorials to learn about the principles of flow cytometry and.Learn at your own speed and repeat as often as needed. Apogee A50 Micro used for enumeration and fluorescence of small particle applications.BD Intro to Flow Training (About 1 hour) BD FACSDiva v6 Software Overview Course. Relieves and avoids occurrence of radiation CELLQUEST is the data acquisition and analysis program from BD Bioscience and operates in the Macintosh environment on the FACSCalibur cytometers. And the Macintosh Computer runs CellQuest v3.3 and Worklist. Cytometer setup and tracking (CST) and FACSDiva 6.1.3 software are used to QC the instrument.

Zetaview Nanoparticle Tracking Analyzer.Introduction to Flow Cytometry II (J. A sampling of our self-paced training courses is listed below. Videos, such as maintenance procedures, allow you to preview a procedure prior to attempting it yourself.

We have a video tutorial on how to use the iLab scheduler if you need help. You can book your two 1-hr training sessions on the traditional flow cytometer of your choice via iLab (Fortessa/LSR, Attune, or Quanteon). FACSDiva v6 Software Overview Course (BD Biosciences).Instructions for Scheduling Training.

Conventional and Spectral Cytometry Panel Building and Data Analysis - FluoroFinder and FCS Express. Applications of Image Cytometry in Digital Pathology: Using the Vectra Platform with FCS Express to Analyze Immune Cell Response. BD FACSDiva and Accuri Importers.

Becton-Dickinson FACS Melody cell sorter (488nm, 561nm, and 640nm direct diode The BD SORP FACSAria Fusion cell sorter consists of 355nm, 405nm, 488nm, 561nm, and 637nm lasers, providing 20 parameters. Becton-Dickinson SORP FACSAria Fusion high performance with ACDU, fixed alignment gel-coupled cuvette cell sorter. Becton-Dickinson High Throughput Sampler (HTS) capable of acquiring samples from 96-well or384-well microtiter plates on the LSRFortessa.

Consultation:All investigators are encouraged to meet with the Scientific Director of the Flow Cytometry Core to discuss their experiment prior to ordering antibodies and other reagents. Histogram Software (Apogee Flow Systems)SpectroFlo, FACSDIVA and FACSChorus are for acquisition only.Analysis workstations and software training are available for researchers. Samples can easily be acquired from 96 well plate format or single tube mode.The Core provides data analysis with the following software: Cytek Aurora spectral flow cytometer equipped with five lasers (355nm, 405nm, 488nm, 561nm and 640 nm excitation wavelengths), three scattering channels, can detect up to 64 fluorescence channels. 10x Chromium Controller for single cell partitioning for transcriptome analysis.

Flow Cytometry Applications: A meeting with both Flow Cytometry and Genomics Core directors is mandatory for pilot 10x experiments. Materials available for reference in the Flow CytometryCore lab include two introductory tutorials available in iLab, several textbooks on the basics of flow cytometry and the current protocols in cytometry.In addition, the Core frequently organizes training seminars for various levels of expertise. Core technologists are available forAssistance with sample acquisition, analysis and presentation. The Core can also provide writtenProtocols for staining, fixation and data analysis methods.

Sample preparation and single cell partitioning using the 10x Chromium Controller Measurements of soluble factors in biological fluids and cell culture supernatants using bead-based 45 color cell surface and/or intracellular (cytokine) immunophenotyping. Functional assays utilizing fluorescent probes such as quantification of mitochondrial organelleFunction, calcium influx and apoptosis, and quantification of expressible fluorescent proteins.

Data can beImported/exported as FCS 2.0, 3.0, or 3.1 when using FACSDIVA. BDFACSDIVA Software is used to acquire data on the Fortessa and Aria II. Micro SpectroFloВ® is used on the Cytek Aurora. Extracellular vesicle enumeration and characterization.Data is acquired as FCS 3.1 using Histogram Software on the Apogee A50.

This cell sorter has a capability of up to 4-way bulk sorting, single-cell plate index sorting, multi-functional temperature control for sample injection and collection. The sorter is enclosed in a Class II Type A2 biosafety cabinet (BSC), designed to offer personnel, product, and environmental protection from potentially aerosolized biological hazards. Users can findIntroductory materials, best practices and protocols on our Links & Resources page.The BD SORP FACSAria Fusion, 20 parameter, 4-way cell sorter is equipped with 355nm, 405nm, 488nm, 561nm, and 637nm laser lines. Getting Started with the Flow Cytometry Core:Please visit our iLab page to schedule services.

Temperature control system for chilled (5oC) collection devices (tubes and multi-well plates)The Apogee A50- MicroВ is specifically designed for measuring sub-micron biological particles. Temperature controlled chamber for sample loading (RT, 4oC, 37oC) Accepts various sizes of sample loading tubes Automated Cell Deposition Unit (ACDU) allows for multi-well plate and slide sorting

Spectral flow cytometry technology has propelled over the past years and offers several key advantages compared to conventional flow cytometry. The optical filters detect the following most commonly used fluorophores: ApogeeConfig (View PDF)CytekTM Aurora spectral cell analyzer housed in the Flow Cytometry Core is equipped with five lasers (355nm: 20mW 405nm:100mW 488nm:50mW 561nm:50mW 640nm:80mW. We are currently one the very few institutes in the USA who offers this service.Our Apogee A50 Micro consists of a 488nm laser, providing 6 parameters. ItвЂ™s only a matter of time before new microparticle-based diagnostic and prognostic tests will be developed and become part of standard clinical care, a progress that was previously hampered by technical challenges of how to enumerate and characterize these microvesicles.The Apogee A50- MicroВ provides accurate analysis and fluorescence measurements of:With a detection limit of >100nm and >10nm resolution, a technical ingenuity lacking in standard flow cytometers, the Apogee Micro Flow Cytometer is designed for analysis of microparticles. Emerging evidence shows that microparticles play critical role in a growing list of processes such as inflammation, infection, immune responses, thrombosis, cancer metastasis and angiogenesis.

An autofluorescence extraction function allows better resolution of dim signals by subtraction of high autofluorescence that is inherent to some cells such as macrophages, epithelial and endothelial cells. Vacuum fluidics allow quantification of absolute numbers. A short-wavelength (violet laser) side scatter detector makes enumeration and immunophenotyping of microparticles possible on the Aurora. The optical configuration is filterless, making the instrument user-friendlier and eliminates errors caused by potential wrong settings. The Aurora utilizes avalanche photodiodes resulting in higher resolution, sensitivity, and linear signals compared to conventional systems. While this is very useful and has been the standard in flow cytometry over the past decades, spectral flow cytometry provides the full fluorescence emission spectrum measured on each cell, enabling easy separation of complex mixtures of fluorochrome combinations that are challenging to co-detect using traditional flow cytometry.

Facsdiva Tutorial Download Word File

Access the Lerner Flow Cytometry Core (CWRU) (View PDF) Flow Cytometry Core iLab GuideВ (View PDF) 10 Simple Clicks to Schedule Time on the Flow Cytometer (Download Word File) Immunophenotyping Methods and Protocols (View PDF)

Relevance of Antibody Validation and Titration for Flow Cytometry (View PDF) Detection of Intracellular Cytokines by Flow Cytometry (View PDF) FlowJo Licensing through Core (View PDF)

Great Lakes International Imaging and Flow Cytometry Association (GLIIFCA) holds an annual meeting in the fall of each year and sponsors this website to serve as a clearingHouse for information of interest to researchers in the Great Lakes region. FluoroFinder is an alternative fluorescence spectrum viewer. Thermo Fisher Scientific's Fluorescence SpetraViewer provides a viewer for selecting fluorescentMarkers and assessing spillover estimates. Visit Washington State University's Taxonomic Key Program website forComparative analysis of the specificity of mAbs specific for leukocyte differentiation molecules